Research Fellow - Marasco Lab (Job ID: 33982)
Description: Our group at the Dana-Farber Cancer Institute is looking for an exceptional Postdoctoral Research Fellow to conduct research on COVID in the area humoral cross-reactivity to circulating coronaviruses and pan-coronavirus vaccines. The project will explore the hypothesis in two parts; 1), that prior immunity against circulating coronaviruses can result in the development of antibodies that cross-bind and neutralize both endemic coronavirus and SARS-CoV-2. Utilizing our large COVID-19 convalescent patient cohort in combination with a cohort collected pre-pandemic, the primary goal of this project is to provide evidence of these cross-reactive antibodies and to establish the molecular basis by which coronavirus cross immunity arises through the identification of common spike epitopes. 2), we have established a humanized mouse model that will allow us to develop mosaic spike based pan-coronavirus vaccines based on the above principles. We will analyze 10X genomics and LibraSeq to perform in depth analysis of the B cells to understand the molecular basis of immune imprinting and memory.
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned innovative researchers, dedicated clinicians and professionals, and allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients; we conduct research that advances treatment; educate tomorrow's researchers/ physician; reach out to underserved members of our community; and work with amazing partners, including other Harvard Medical School-affiliated hospitals.
Responsibilities: Our lab at Dana-Farber Cancer Institute is seeking a motivated and talented fellow who will contribute to discovery and translational research. The major focus of this specific position will be for a very talented and committed fellow to perform serologic and molecular studies, develop and execute biological assays, interpret the results, analyze the data, and support further research activities. We are looking to expand our laboratory of creative, collaborative, yet independent thinkers, with high scientific rigor and enthusiasm who are most likely to succeed within our team. Mentorship of the candidate will focus on the development and publication of high-impact research projects and preparing the fellow for a career as an independent academic investigator or for a position in the biomedical industry.
Qualifications:
Description: Our group at the Dana-Farber Cancer Institute is looking for an exceptional Postdoctoral Research Fellow to conduct research on COVID in the area humoral cross-reactivity to circulating coronaviruses and pan-coronavirus vaccines. The project will explore the hypothesis in two parts; 1), that prior immunity against circulating coronaviruses can result in the development of antibodies that cross-bind and neutralize both endemic coronavirus and SARS-CoV-2. Utilizing our large COVID-19 convalescent patient cohort in combination with a cohort collected pre-pandemic, the primary goal of this project is to provide evidence of these cross-reactive antibodies and to establish the molecular basis by which coronavirus cross immunity arises through the identification of common spike epitopes. 2), we have established a humanized mouse model that will allow us to develop mosaic spike based pan-coronavirus vaccines based on the above principles. We will analyze 10X genomics and LibraSeq to perform in depth analysis of the B cells to understand the molecular basis of immune imprinting and memory.
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned innovative researchers, dedicated clinicians and professionals, and allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients; we conduct research that advances treatment; educate tomorrow's researchers/ physician; reach out to underserved members of our community; and work with amazing partners, including other Harvard Medical School-affiliated hospitals.
Responsibilities: Our lab at Dana-Farber Cancer Institute is seeking a motivated and talented fellow who will contribute to discovery and translational research. The major focus of this specific position will be for a very talented and committed fellow to perform serologic and molecular studies, develop and execute biological assays, interpret the results, analyze the data, and support further research activities. We are looking to expand our laboratory of creative, collaborative, yet independent thinkers, with high scientific rigor and enthusiasm who are most likely to succeed within our team. Mentorship of the candidate will focus on the development and publication of high-impact research projects and preparing the fellow for a career as an independent academic investigator or for a position in the biomedical industry.
- Execute serologic studies to quantify cross-reactivity in COVID-19 convalescent and control plasma samples
- Purify plasma IgGs for in vitro and in vivo characterization and neuralization experiments
- Conduct cell-based and molecular biology research aimed at isolating immune B cells from the two human cohorts for the discovery of cross-reactive antibodies.
- Production and characterization of these mAbs for epitope binning, kinetic binding measurements and neutralization activity
- Maintaining accurate records, writing reports, and preparing/giving presentations.
Qualifications:
- PhD, MD, MD/PhD or doctoral equivalent with interest in the biological sciences
- A strong background in immunology, molecular biology and cell biology
- Excellent oral and written communication skills
- Ability to perform both self-directed and guided research
- Effectively work as part of an interdisciplinary team of scientists
Marasco Lab Clinical Research Coordinator (internship/co-op)
The Marasco Lab at the Dana-Farber Cancer Institute is looking for motivated students to work as Clinical Research Coordinators (CRC) for our influenza vaccination study. This study aims to evaluate the immune response to vaccination and to determine how aging affects this response. CRCs will be responsible for obtaining informed consent on these non-treatment studies, explaining the purpose and requirements of the studies to prospective participants, completing eligibility screening, registration of eligible participants, collecting survey and other patient information, and ensuring timely collection of protocol related samples. Both on/off-site versions of this position are available, however for students who are able to be onsite, an added bonus for this role is the opportunity to work 1:1 with donors.
Required responsibilities:
Possible responsibilities depending on time available and on/off site status:
Qualifications:
Excellent organization and communications skills required. Strong interpersonal skills; ability to effectively interact with staff and study participants. Must be detail oriented and have the ability to follow-through. Ability to effectively manage time and prioritize workload. Must practice discretion and adhere to hospital confidentiality guidelines at all times. Must have computer skills including the use of Microsoft Office. No previous experience is required, though experience in a clinical setting is preferred.
Hours:
Ideally students will be able to commit 15-20 hours per week for this position, however this can be discussed on a case-by-case basis. A large part of this position involves communicating with study participants and we anticipate that the majority of the work will be completed during standard working hours. Additionally, on-site availability is not required for this position, however it is preferred and for those able, on-site work will typically be from 8:00 am – 1 pm.
The Marasco Lab at the Dana-Farber Cancer Institute is looking for motivated students to work as Clinical Research Coordinators (CRC) for our influenza vaccination study. This study aims to evaluate the immune response to vaccination and to determine how aging affects this response. CRCs will be responsible for obtaining informed consent on these non-treatment studies, explaining the purpose and requirements of the studies to prospective participants, completing eligibility screening, registration of eligible participants, collecting survey and other patient information, and ensuring timely collection of protocol related samples. Both on/off-site versions of this position are available, however for students who are able to be onsite, an added bonus for this role is the opportunity to work 1:1 with donors.
Required responsibilities:
- Develop appropriate excel tables to track tasks and appointments
- Assess recruiting data base(s) for potential participants
- Make initial contact with potential participant (phone and email).
- By phone, provide details of the consent and study process, collect study-related information, and schedule clinical visits with participants.
- Troubleshoot problems as they occur.
- Maintain detailed records of participant visits and study/sample-related information.
Possible responsibilities depending on time available and on/off site status:
- Meet and escort donors and coordinate sample collection with phlebotomist and Marasco lab personnel.
- Serve as an in-person knowledge source for details of study and study consent process.
- Administer surveys to study participants to collect critical study-related information.
Qualifications:
Excellent organization and communications skills required. Strong interpersonal skills; ability to effectively interact with staff and study participants. Must be detail oriented and have the ability to follow-through. Ability to effectively manage time and prioritize workload. Must practice discretion and adhere to hospital confidentiality guidelines at all times. Must have computer skills including the use of Microsoft Office. No previous experience is required, though experience in a clinical setting is preferred.
Hours:
Ideally students will be able to commit 15-20 hours per week for this position, however this can be discussed on a case-by-case basis. A large part of this position involves communicating with study participants and we anticipate that the majority of the work will be completed during standard working hours. Additionally, on-site availability is not required for this position, however it is preferred and for those able, on-site work will typically be from 8:00 am – 1 pm.
Research Fellow - Marasco Lab (Job ID: 2022-28990)
Description:
A postdoctoral fellow position in the Marasco lab in the Department of Cancer Immunology and Virology is available to join our human antibody discovery and chimeric antigen receptor (CAR) T-cell cancer therapy programs. This fellow will lead our efforts in developing immune restoring (IR) CAR T cells and examining the combinatorial effect of CAR T with expression of the immune modulator antibodies on inhibiting tumor growth in vitro and in vivo in ovarian cancer (OvCA). Working with a team of dedicated research staff including computational, cellular, molecular and structural biologists and virologists, this fellow will be responsible for designing and executing in vitro and in vivo experiments for their designated disease area.
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and emerging infectious diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
Responsibilities:
The postdoctoral fellow will generate cancer target specific CAR constructs, create CAR T-cells, carry out in vitro assays to test and optimize their biological activities, including generating appropriate tumor target cell lines, optimizing CAR T proliferation conditions, setting up in vitro target cell killing assays, characterizing CAR T cells’ responses to stimulation by target cells such as degranulation, cytokine production, cellular phosphorylation levels of intracellular signaling domains and activation induced cell death. The fellow is expected to set up appropriate tumor model(s) in humanized mice and perform in vivo experiments to evaluate therapeutic potential of specific IR CAR T-cells for the intended cancer target(s). In addition to monitoring tumor growth and metastases, the fellow will assess the activity of CAR T cells (proliferation, exhaustion etc.), and analyze tumor microenvironment by transcriptional profiling using scRNAseq and other molecular means as well as multiparameter flow cytometry/immunohistochemistry. While we are not a computational biology laboratory, support for scRNAseq will be provided but a candidate should become familiar with this field. The candidate must be a self-starter and highly motivated team player. The fellow is expected to work independently, well versed in the related literature, and contribute/lead intellectually to their research program. The team member will also be responsible for providing regular written and oral presentations. The successful candidate must demonstrate excellent writing and verbal communications skills.
Qualifications:
Ph.D. degree with documented research experience in molecular biology and immunology, especially in antibody discovery and/or CAR T cell therapy. Experiences in cancer animal model and relevant molecular, biochemical and immunological techniques are preferred. We are particular interested in the candidates with prior research experience in the area of cellular immunology, gene-editing, and/or specializing in mouse surgery and establishing human immune system reconstructed mouse models. The individual is also expected to be able to parallel processing (good time management skills), well organized, and pay careful attention to experimental details. While supervisory experience will be a plus, the candidate should expect to be fully involved in hands-on bench work.
Description:
A postdoctoral fellow position in the Marasco lab in the Department of Cancer Immunology and Virology is available to join our human antibody discovery and chimeric antigen receptor (CAR) T-cell cancer therapy programs. This fellow will lead our efforts in developing immune restoring (IR) CAR T cells and examining the combinatorial effect of CAR T with expression of the immune modulator antibodies on inhibiting tumor growth in vitro and in vivo in ovarian cancer (OvCA). Working with a team of dedicated research staff including computational, cellular, molecular and structural biologists and virologists, this fellow will be responsible for designing and executing in vitro and in vivo experiments for their designated disease area.
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and emerging infectious diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
Responsibilities:
The postdoctoral fellow will generate cancer target specific CAR constructs, create CAR T-cells, carry out in vitro assays to test and optimize their biological activities, including generating appropriate tumor target cell lines, optimizing CAR T proliferation conditions, setting up in vitro target cell killing assays, characterizing CAR T cells’ responses to stimulation by target cells such as degranulation, cytokine production, cellular phosphorylation levels of intracellular signaling domains and activation induced cell death. The fellow is expected to set up appropriate tumor model(s) in humanized mice and perform in vivo experiments to evaluate therapeutic potential of specific IR CAR T-cells for the intended cancer target(s). In addition to monitoring tumor growth and metastases, the fellow will assess the activity of CAR T cells (proliferation, exhaustion etc.), and analyze tumor microenvironment by transcriptional profiling using scRNAseq and other molecular means as well as multiparameter flow cytometry/immunohistochemistry. While we are not a computational biology laboratory, support for scRNAseq will be provided but a candidate should become familiar with this field. The candidate must be a self-starter and highly motivated team player. The fellow is expected to work independently, well versed in the related literature, and contribute/lead intellectually to their research program. The team member will also be responsible for providing regular written and oral presentations. The successful candidate must demonstrate excellent writing and verbal communications skills.
Qualifications:
Ph.D. degree with documented research experience in molecular biology and immunology, especially in antibody discovery and/or CAR T cell therapy. Experiences in cancer animal model and relevant molecular, biochemical and immunological techniques are preferred. We are particular interested in the candidates with prior research experience in the area of cellular immunology, gene-editing, and/or specializing in mouse surgery and establishing human immune system reconstructed mouse models. The individual is also expected to be able to parallel processing (good time management skills), well organized, and pay careful attention to experimental details. While supervisory experience will be a plus, the candidate should expect to be fully involved in hands-on bench work.
Research Fellow - Marasco Lab (Job ID: 2022-27339)
Description:
Exciting opportunity for an exceptional post-doc applicant to be co-mentored by Drs. Hemberg (computational biology) and Marasco (infectious diseases) in the area of infectious diseases research. The Hemberg group, formerly at the Wellcome Sanger Institute (https://www.sanger.ac.uk/group/hemberg-group/) has recently relocated to the Evergrande Center for Immunological Diseases (https://evergrande.hms.harvard.edu/home) at the Department of Neurology at Brigham and Women’s Hospital and the Department of Immunology at Harvard Medical School in Boston. This location offers unique opportunities in terms of scientific collaborations in a highly dynamic and interdisciplinary environment.
The Marasco lab at Dana-Farber Cancer Institute (www.marascolab.org) is leading the bench work on two related research projects to understand the human antibody response to emerging pathogens. Importantly, the Hemberg and Marasco labs are located within short walking distance from each other. We have recently initiated a collaboration related to the use of single-cell RNA-seq to understand human B cells and antibody responses to influenza and COVID vaccines.
Responsibilities
The funding available is for an initial appointment over three years and the starting date is flexible. The candidate will be expected to carry out research (both independently and as part of a team), participate in group discussions, and write research proposals and manuscripts. Successful candidates should demonstrate a track record of research achievements and have excellent communication skills.
Qualifications
Those with a background in related areas such as computer science, physics, chemistry, statistics and mathematics are encouraged to apply. The successful candidate will have strong quantitative skills, and a good knowledge of R, python and/or other programming languages. Background knowledge in immunology, genomics and molecular biology is desirable, but not required.
Description:
Exciting opportunity for an exceptional post-doc applicant to be co-mentored by Drs. Hemberg (computational biology) and Marasco (infectious diseases) in the area of infectious diseases research. The Hemberg group, formerly at the Wellcome Sanger Institute (https://www.sanger.ac.uk/group/hemberg-group/) has recently relocated to the Evergrande Center for Immunological Diseases (https://evergrande.hms.harvard.edu/home) at the Department of Neurology at Brigham and Women’s Hospital and the Department of Immunology at Harvard Medical School in Boston. This location offers unique opportunities in terms of scientific collaborations in a highly dynamic and interdisciplinary environment.
The Marasco lab at Dana-Farber Cancer Institute (www.marascolab.org) is leading the bench work on two related research projects to understand the human antibody response to emerging pathogens. Importantly, the Hemberg and Marasco labs are located within short walking distance from each other. We have recently initiated a collaboration related to the use of single-cell RNA-seq to understand human B cells and antibody responses to influenza and COVID vaccines.
Responsibilities
The funding available is for an initial appointment over three years and the starting date is flexible. The candidate will be expected to carry out research (both independently and as part of a team), participate in group discussions, and write research proposals and manuscripts. Successful candidates should demonstrate a track record of research achievements and have excellent communication skills.
Qualifications
Those with a background in related areas such as computer science, physics, chemistry, statistics and mathematics are encouraged to apply. The successful candidate will have strong quantitative skills, and a good knowledge of R, python and/or other programming languages. Background knowledge in immunology, genomics and molecular biology is desirable, but not required.
Research Technician - Marasco Lab (Job ID: 2021-25366)
Description:
A lab technician position is available for a highly motivated and experienced individual who is interested in hands-on bench research in the area of therapeutic antibody discovery and engineering. This individual will be an integral member of our research team and will work with more senior members of the lab on different research projects including identifying murine/human antibodies and developing new therapies for cancer and infectious disease related applications.
As a bench scientist, this position requires basic biochemical, molecular and cell biology skills including day-to-day cell culture, molecular cloning, and various protein production/analysis techniques. There will be opportunities to participate in the development of various immunotherapies, including checkpoint modulating antibodies and CAR T cells, and to conduct biological assays to characterize these therapeutics in vitro and in vivo. Additional work includes performing literature searches, writing up methods and materials, results, figure legends and constructing figures for grant proposals, progress reports, and manuscripts.
This position also shares responsibilities for lab maintenance and operation. Applicant with 1+ years of lab experience with strong background in both molecular and cell biology techniques including production of recombinant proteins in E. coli and mammalian cells as well as subsequent biochemical and immunological analysis are preferred.
Requirements:
Description:
A lab technician position is available for a highly motivated and experienced individual who is interested in hands-on bench research in the area of therapeutic antibody discovery and engineering. This individual will be an integral member of our research team and will work with more senior members of the lab on different research projects including identifying murine/human antibodies and developing new therapies for cancer and infectious disease related applications.
As a bench scientist, this position requires basic biochemical, molecular and cell biology skills including day-to-day cell culture, molecular cloning, and various protein production/analysis techniques. There will be opportunities to participate in the development of various immunotherapies, including checkpoint modulating antibodies and CAR T cells, and to conduct biological assays to characterize these therapeutics in vitro and in vivo. Additional work includes performing literature searches, writing up methods and materials, results, figure legends and constructing figures for grant proposals, progress reports, and manuscripts.
This position also shares responsibilities for lab maintenance and operation. Applicant with 1+ years of lab experience with strong background in both molecular and cell biology techniques including production of recombinant proteins in E. coli and mammalian cells as well as subsequent biochemical and immunological analysis are preferred.
Requirements:
- Standard laboratory techniques and skills (pipetting, cell culture, PCR, plasmid prep, etc.)
- Conduct experiments, analyze experimental data and interpret results for publication
- Maintain inventory of lab supplies/reagents and contribute to maintenance and upkeep of lab equipment
- Keep a detailed notebook of all work-related activities including experimental procedures, results, and conclusions
- Contribute at an intellectual level by remaining up to date on appropriate research publications
- Present data and advances in the field to other members of the lab in both formal and informal settings
- Bachelor's Degree required with a major in biology or a related scientific field preferred
- Minimum of 1 year of related experience
- Experience in molecular biology, protein expression and purification, and cell culture is preferred
- Knowledge of techniques related to immunology, biology or infectious disease research is preferred
- Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.
- Must be a self-starter and a highly motivated team player with excellent attention to detail
- Have the ability to analyze information and use logic to address work-related issues and problems
- Ability to work within a team environment.
Research Technician - Marasco Lab (Job ID: 2021-26156)
Description:
A lab technician position is available for a highly motivated and experienced individual who is interested in hands-on bench research in the area of viral immunology and therapeutic antibody discovery. This individual will be an integral member of our research team and will be mentored by senior members of the lab on different research projects, including identifying murine/human antibodies and developing new therapies for infectious disease related applications.
As a bench scientist, this position requires basic biochemical, molecular and cell biology skills including day-to-day cell culture, molecular cloning, various protein production/analysis techniques. There will be opportunities to contribute to research projects related to humoral cross-reactivity against influenza and COVID-19 infection, as well as the discovery of novel antibodies against Borrelia burgdorferi, the causative agent of Lyme disease. Additional work includes performing literature searches, writing up methods and materials, results, figure legends and constructing figures for grant proposals, progress reports, and manuscripts.
This position also shares responsibilities for lab maintenance and operation. Applicant with 1+ years of lab experience with strong background in both molecular and cell biology techniques including production of recombinant proteins in E. coli and mammalian cells as well as subsequent biochemical and immunological analysis are preferred.
Requirements:
Description:
A lab technician position is available for a highly motivated and experienced individual who is interested in hands-on bench research in the area of viral immunology and therapeutic antibody discovery. This individual will be an integral member of our research team and will be mentored by senior members of the lab on different research projects, including identifying murine/human antibodies and developing new therapies for infectious disease related applications.
As a bench scientist, this position requires basic biochemical, molecular and cell biology skills including day-to-day cell culture, molecular cloning, various protein production/analysis techniques. There will be opportunities to contribute to research projects related to humoral cross-reactivity against influenza and COVID-19 infection, as well as the discovery of novel antibodies against Borrelia burgdorferi, the causative agent of Lyme disease. Additional work includes performing literature searches, writing up methods and materials, results, figure legends and constructing figures for grant proposals, progress reports, and manuscripts.
This position also shares responsibilities for lab maintenance and operation. Applicant with 1+ years of lab experience with strong background in both molecular and cell biology techniques including production of recombinant proteins in E. coli and mammalian cells as well as subsequent biochemical and immunological analysis are preferred.
Requirements:
- Standard laboratory and molecular biology techniques (DNA cloning, PCR, ELISA, plasmid prep, cell culture, etc.)
- Conduct experiments, analyze experimental data and interpret results for publication
- Maintain inventory of lab supplies/reagents and contribute to maintenance and upkeep of lab equipment
- Keep a detailed notebook of all work-related activities including experimental procedures, results, and conclusions
- Contribute at an intellectual level by remaining up to date on appropriate research publications
- Present data and advances in the field to other members of the lab in both formal and informal settings
- Bachelor's Degree required with a major in biology or a related scientific field preferred
- Minimum of 1 year of related experience
- Experience in molecular biology, protein expression and purification, and cell culture is preferred
- Knowledge of techniques related to immunology, biology or infectious disease research is preferred
- Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.
- Must be a self-starter and a highly motivated team player with excellent attention to detail
- Have the ability to analyze information and use logic to address work-related issues and problems
- Ability to work within a team environment.
Post-doctoral Fellow - Marasco Lab (Job ID: 2021-25412)
Overview
Our group at Dana-Farber Cancer Institute is looking for exceptional Post-doctoral Fellow to conduct research in an area of Tick-borne infectious diseases, specifically Lyme Disease. This is an inter-institutional collaborative project involving MIT, Whitehead Institute, Tufts and Dana-Farber Cancer Institute.
The project builds on field studies in which researchers partially vaccinated populations of the white-footed mouse with the outer surface protein A (Osp A) from B. burgdorferi and found that these mice play a critical role in controlling high levels of the Lyme pathogen in the environment. We plan to engineer white-footed mice to carry multiple copies of anti-Osp A neutralizing antibodies against B. burgdorfei, in hopes of disrupting the transmission cycles for decades, substantially reducing the total number of infected ticks and the risk of human exposure.
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned innovative researchers, dedicated clinicians and professionals, and allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients; we conduct research that advances treatment; educate tomorrow's researchers/ physician; reach out to underserved members of our community; and work with amazing partners, including other Harvard Medical School-affiliated hospitals.
Requirements
Our lab at Dana-Farber Cancer Institute is seeking a motivated and talented scientist who will contribute to discovery and translational research. The major focus of this specific position will be for a very talented and committed scientist to perform antibody discovery and cellular engineering, develop and execute biological assays, interpret the results, analyze the data, and support further research activities. We are looking to expand our laboratory of creative, collaborative, yet independent thinkers, with high scientific rigor and enthusiasm who are most likely to succeed within our team. Mentorship of the candidate will focus on the development and publication of high-impact research projects and preparing the fellow for a career as an independent academic investigator or for a position in the biomedical industry.
Specific responsibilities include:
Overview
Our group at Dana-Farber Cancer Institute is looking for exceptional Post-doctoral Fellow to conduct research in an area of Tick-borne infectious diseases, specifically Lyme Disease. This is an inter-institutional collaborative project involving MIT, Whitehead Institute, Tufts and Dana-Farber Cancer Institute.
The project builds on field studies in which researchers partially vaccinated populations of the white-footed mouse with the outer surface protein A (Osp A) from B. burgdorferi and found that these mice play a critical role in controlling high levels of the Lyme pathogen in the environment. We plan to engineer white-footed mice to carry multiple copies of anti-Osp A neutralizing antibodies against B. burgdorfei, in hopes of disrupting the transmission cycles for decades, substantially reducing the total number of infected ticks and the risk of human exposure.
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned innovative researchers, dedicated clinicians and professionals, and allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients; we conduct research that advances treatment; educate tomorrow's researchers/ physician; reach out to underserved members of our community; and work with amazing partners, including other Harvard Medical School-affiliated hospitals.
Requirements
Our lab at Dana-Farber Cancer Institute is seeking a motivated and talented scientist who will contribute to discovery and translational research. The major focus of this specific position will be for a very talented and committed scientist to perform antibody discovery and cellular engineering, develop and execute biological assays, interpret the results, analyze the data, and support further research activities. We are looking to expand our laboratory of creative, collaborative, yet independent thinkers, with high scientific rigor and enthusiasm who are most likely to succeed within our team. Mentorship of the candidate will focus on the development and publication of high-impact research projects and preparing the fellow for a career as an independent academic investigator or for a position in the biomedical industry.
Specific responsibilities include:
- Conducting cell-based and molecular biology research aimed at isolating immune B cells from immunized mice for the eventual discovery of anti-Osp A monoclonal antibodies (mAbs). Our laboratory utilizes several technologies to isolate these mAb including single cell plasmablast and/or memory B cells cloning as well as use of phage and yeast display platforms.
- Production and characterization of these mAbs for epitope binning, kinetic binding measurements and neutralization activity
- Performing preclinical studies in mouse models Lyme disease.
- Maintaining accurate records, writing reports, and preparing/giving presentations.
- PhD, MD, or MD/PhD with interest in the biological sciences
- A strong background in immunology, molecular biology and cell biology
- Excellent oral and written communication skills
- Ability to perform both self-directed and guided research
- Effectively work as part of an interdisciplinary team of scientists
JOIN US
The Marasco Lab offers exciting research opportunities in human antibody engineering and regenerative medicine. With our numerous tools including state-of-the-art humanized mouse models, we are making discoveries in B cell development, host immunity and vaccine design for both infectious disease and cancer immunotherapy applications. Our research team focuses on identification of clinically relevant human monoclonal antibodies and studies of their mechanism of action. Our humanized mouse models are also being used to investigate the pluripotency of adult stem cells with applications in the field of regenerative medicine.
Working in Dr. Marasco's laboratory provides exposure to the most recent developments in the field of antibody engineering, cancer immunotherapy, and adult stem cell biology. We are always looking for highly motivated post-doctoral fellows and graduate students to join our research team, particularly those individuals with high intellect who are also creative thinkers.
Applicants should have a strong interest in human translational research. Job openings are posted with DFCI Human Resources. Interested applicants should address their cover letter to:
Wayne A. Marasco, M.D., Ph.D.
Department of Cancer Immunology & Virology
Dana-Farber Cancer Institute
450 Brookline Avenue
Boston, MA 02215
Working in Dr. Marasco's laboratory provides exposure to the most recent developments in the field of antibody engineering, cancer immunotherapy, and adult stem cell biology. We are always looking for highly motivated post-doctoral fellows and graduate students to join our research team, particularly those individuals with high intellect who are also creative thinkers.
Applicants should have a strong interest in human translational research. Job openings are posted with DFCI Human Resources. Interested applicants should address their cover letter to:
Wayne A. Marasco, M.D., Ph.D.
Department of Cancer Immunology & Virology
Dana-Farber Cancer Institute
450 Brookline Avenue
Boston, MA 02215
Current Openings
If you are interested in joining the lab, please send your CV and cover letter to MarascoLab@dfci.harvard.edu.