ANTIBODY ENGINEERING
We are leaders in the field of human antibody engineering and have been involved in many of the seminal discoveries in the field. We are experts in phage, yeast and lentivirus display platforms and panning procedures, human antibody library constructions, Fc engineering, humanization procedures and targeted siRNA delivery to name a few. We have constructed human antibody libraries with tens of billion members and have successfully isolated therapeutic human antibodies against over two dozen targets. We have also developed and utilize antibody expression systems in bacteria, mammalian, yeast and insect cells. Students and post-doctoral fellows join the Marasco lab to become skilled artisans in the field under Dr. Marasco’s mentorship. An overview of some of our antibody engineering tools can be found here.
Gennari F, Lopes L, Verhoeyen E, Marasco W, Collins MK. Single-chain antibodies that target lentiviral vectors to MHC class II on antigen-presenting cells. Hum Gene Ther. 2009 Jun;20(6):554-62. PMID: 19260768.
Taube R, Zhu Q, Xu C, Diaz-Griffero F, Sui J, Kamau E, Dwyer M, Aird D, Marasco WA. Lentivirus display: stable expression of human antibodies on the surface of human cells and virus particles. PLoS One. 2008 Sep 11;3(9):e3181. PMID: 18784843. PMCID: PMC2527531.
Marasco WA, Sui J.The growth and potential of human antiviral monoclonal antibody therapeutics. Nat Biotechnol. 2007 Dec;25(12):1421-34. PMID: 18066039.
Xu C, Sui J, Tao H, Zhu Q, Marasco WA. Human anti-CXCR4 antibodies undergo VH replacement, exhibit functional V-region sulfation, and define CXCR4 antigenic heterogeneity. J Immunol. 2007 Aug 15;179(4):2408-18. PMID: 17675502.
Bal HP, Cheng J, Murakami A, Tallarico AS, Wang W, Zhou D, Vasicek TJ, Marasco WA. GITR overexpression on CD4+CD25+ HTLV-1 transformed cells: detection by massively parallel signature sequencing. Biochem Biophys Res Commun. 2005 Jul 1;332(2):569-84. PMID: 15896717.
Marasco WA. Therapeutic antibody gene transfer. Nat Biotechnol. 2005 May;23(5):551-2. PMID: 15877069.
Busch A, Marasco WA, Doebis C, Volk HD, Seifert M. MHC class I manipulation on cell surfaces by gene transfer of anti-MHC class I intrabodies – a tool for decreased immunogenicity of allogeneic tissue and cell transplants. Methods. 2004 Oct;34(2):240-9. PMID: 15312677.
Beyer F, Doebis C, Busch A, Ritter T, Mhashilkar A, Marasco W, Laube H, Volk HD, Seifert M. Decline of surface MHC I by adenoviral gene transfer of anti-MHC I intrabodies in human endothelial cells - new perspectives for the generation of universal donor cells for tissue transplantation. J Gene Med. 2004 Jun;6(6):616-23. PMID: 15170732.
Zhu Q, Ricardo RR, Zhang L, Ogueta SB, Agrawal RS, Dzau VJ, Marasco WA. Development of constitutive and inducible self-inactivating lentiviral vectors and their application in cardiovascular gene transfer. Gene Ther Mol Biol. 2004 Mar;8:91-102.
Gennari F, Mehta S, Wang Y, St Clair Tallarico A, Palu G, Marasco WA. Direct phage to intrabody screening (DPIS): demonstration by isolation of cytosolic intrabodies against the TES1 site of Epstein Barr virus latent membrane protein 1 (LMP1) that block NF-kB transactivation. J Mol Biol. 2004 Jan 2;335(1):193-207. PMID: 14659750.
Sui J, Bai J, St Clair Tallarico A, Xu C, Marasco WA. Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection. Eur J Biochem. 2003 Nov;270(22):4497-506. PMID: 14622278.
Mhashilkar AM, Doebis C, Seifert M, Busch A, Zani C, Soo Hoo J, Nagy M, Ritter T, Volk HD, Marasco WA. Intrabody-mediated phenotypic knockout of major histocompatibility complex class I expression in human and monkey cell lines and in primary human keratinocytes. Gene Ther. 2002 Mar;9(5):307-19. PMID: 11938450.
Gennari F, Lopes L, Verhoeyen E, Marasco W, Collins MK. Single-chain antibodies that target lentiviral vectors to MHC class II on antigen-presenting cells. Hum Gene Ther. 2009 Jun;20(6):554-62. PMID: 19260768.
Taube R, Zhu Q, Xu C, Diaz-Griffero F, Sui J, Kamau E, Dwyer M, Aird D, Marasco WA. Lentivirus display: stable expression of human antibodies on the surface of human cells and virus particles. PLoS One. 2008 Sep 11;3(9):e3181. PMID: 18784843. PMCID: PMC2527531.
Marasco WA, Sui J.The growth and potential of human antiviral monoclonal antibody therapeutics. Nat Biotechnol. 2007 Dec;25(12):1421-34. PMID: 18066039.
Xu C, Sui J, Tao H, Zhu Q, Marasco WA. Human anti-CXCR4 antibodies undergo VH replacement, exhibit functional V-region sulfation, and define CXCR4 antigenic heterogeneity. J Immunol. 2007 Aug 15;179(4):2408-18. PMID: 17675502.
Bal HP, Cheng J, Murakami A, Tallarico AS, Wang W, Zhou D, Vasicek TJ, Marasco WA. GITR overexpression on CD4+CD25+ HTLV-1 transformed cells: detection by massively parallel signature sequencing. Biochem Biophys Res Commun. 2005 Jul 1;332(2):569-84. PMID: 15896717.
Marasco WA. Therapeutic antibody gene transfer. Nat Biotechnol. 2005 May;23(5):551-2. PMID: 15877069.
Busch A, Marasco WA, Doebis C, Volk HD, Seifert M. MHC class I manipulation on cell surfaces by gene transfer of anti-MHC class I intrabodies – a tool for decreased immunogenicity of allogeneic tissue and cell transplants. Methods. 2004 Oct;34(2):240-9. PMID: 15312677.
Beyer F, Doebis C, Busch A, Ritter T, Mhashilkar A, Marasco W, Laube H, Volk HD, Seifert M. Decline of surface MHC I by adenoviral gene transfer of anti-MHC I intrabodies in human endothelial cells - new perspectives for the generation of universal donor cells for tissue transplantation. J Gene Med. 2004 Jun;6(6):616-23. PMID: 15170732.
Zhu Q, Ricardo RR, Zhang L, Ogueta SB, Agrawal RS, Dzau VJ, Marasco WA. Development of constitutive and inducible self-inactivating lentiviral vectors and their application in cardiovascular gene transfer. Gene Ther Mol Biol. 2004 Mar;8:91-102.
Gennari F, Mehta S, Wang Y, St Clair Tallarico A, Palu G, Marasco WA. Direct phage to intrabody screening (DPIS): demonstration by isolation of cytosolic intrabodies against the TES1 site of Epstein Barr virus latent membrane protein 1 (LMP1) that block NF-kB transactivation. J Mol Biol. 2004 Jan 2;335(1):193-207. PMID: 14659750.
Sui J, Bai J, St Clair Tallarico A, Xu C, Marasco WA. Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection. Eur J Biochem. 2003 Nov;270(22):4497-506. PMID: 14622278.
Mhashilkar AM, Doebis C, Seifert M, Busch A, Zani C, Soo Hoo J, Nagy M, Ritter T, Volk HD, Marasco WA. Intrabody-mediated phenotypic knockout of major histocompatibility complex class I expression in human and monkey cell lines and in primary human keratinocytes. Gene Ther. 2002 Mar;9(5):307-19. PMID: 11938450.